In IsoMS-Quant, a mass spectral peak pair found in the metabolite-intensity table generated by IsoMS and Zero-fill is searched against the raw LC-MS data to find all neighboring mass spectra where the same peak pair is continuously detected. The chromatographic peaks of the light-labeled and heavy-labeled metabolites in the pair are constructed and their peak areas are determined for peak ratio measurement.
The IsoMS-Quant program written in R (Windows) can be freely downloaded for non-commercial use (download IsoMS-Quant).
The User Manual (download the PDF file) provides the instructions on installation and use of the IsoMS-Quant program..
